Plasmid

Part:BBa_K510001:Design

Designed by: Fernando Govantes   Group: iGEM11_UPO-Sevilla   (2011-09-16)

pUC18SfiI-miniTn7BB-Cm


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4479
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4479
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4485
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4479
    Illegal BglII site found at 3051
    Illegal BglII site found at 3322
    Illegal XhoI site found at 3408
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4479
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4479
    Illegal XbaI site found at 4494
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1681
    Illegal SapI.rc site found at 2763


Design Notes

NcoI and SphI sites were added at the ends of the chloramphenicol resistance cassette to facilitate cloning


Source

This construct is the result of replacing the gentamycin resistance cassette in pUC18SfiI-miniTn7BB-Gm (K510000) by a chloramphenicol resistance cassette obtained from pBS1C3 by PCR amplification

References